中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5216-5221.doi: 10.3969/j.issn. 2095-4344.2012.28. 018

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

小分子量透明质酸促进星形胶质细胞中脑源性神经营养因子和 血管内皮生长因子的表达

胡 星，王 军，韦 峰，刘忠军，姜 亮，刘晓光   

  1. 北京大学第三医院，北京市 100191
  • 收稿日期:2012-04-01 修回日期:2012-05-09 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 刘晓光，博士生导师，教授，主任医师，；北京大学第三医院骨科，北京市海淀区 100191 xglius@ vip.sina.com.cn
  • 作者简介:胡星★，女，1983年生，安徽省怀宁县人，北京大学第三医院在读硕士，主要从事脊髓损伤的康复临床与基础研究。 huxing@ bjmu.edu.cn

Effect of small molecular-weight hyaluronan on up-regulating expression of brain-derived neurotrophic factor and vascular endothelial growth factor in astrocytes

Hu Xing, Wang Jun, Wei Feng, Liu Zhong-jun, Jiang Liang, Liu Xiao-guang   

  1. Peking University Third Hospital, Beijing 100191, China
  • Received:2012-04-01 Revised:2012-05-09 Online:2012-07-08 Published:2012-07-08
  • Contact: Liu Xiao-guang, Doctoral supervisor, Professor, Chief physician, Peking University Third Hospital, Beijing 100191, China xglius@ vip.sina.com.cn
  • About author:Hu Xing★, Studying for master’s degree, Peking University Third Hospital, Beijing 100191, China huxing@bjmu.edu.cn

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511

被引次数

3

摘要：

背景：国外研究表明透明质酸可以促进脊髓损伤后大鼠运动功能的改善，但其机制尚不清楚。
目的：观察透明质酸对星形胶质细胞营养因子表达的影响。
方法：无菌条件下分离SD大鼠脑组织星形胶质细胞进行原代培养，对培养第3代细胞采用免疫化学方法对其鉴定。对第4代细胞给予透明质酸(相对分子质量125 000~175 000)作用星形胶质细胞24 h，并设置对照组。
结果与结论：免疫化学方法鉴定原代培养星形胶质细胞阳性率近100%。Western blot和RT-PCR分析结果显示，胶质细胞在质量浓度100，1 000 mg/L 透明质酸作用下同10，1 mg/L透明质酸组和对照组比较，脑源性神经营养因子和血管内皮生长因子表达明显升高(P < 0.05)；免疫荧光双标显示在质量浓度1 000 mg/L 透明质酸作用下，同对照组相比，胶质细胞脑源性神经营养因子和血管内皮生长因子表达明显增加。证实小分子量透明质酸可上调星形胶质细胞中脑源性神经营养因子和血管内皮生长因子的表达。

关键词: 星形胶质细胞, 透明质酸, 神经营养因子, 脊髓修复, 组织工程

Abstract:

BACKGROUND: Foreign studies have shown that hyaluronan can contribute to the improvement of motor function after spinal cord injury in rats, but its mechanism is unclear.
OBJECTIVE: To study the effect of hyaluronan on expression of neurotrophic factors in astrocytes.
METHODS: Cultured astrocytes were obtained from the brain of SD rats under sterile conditions. Passage 3 cells were identified by immunochemistry method. Passage 4 cells were treated with hyaluronan (Mr:125 000-175 000) for 24 hours, and control group was set.
RESULTS AND CONCLUSION: The results of immunofluoresence showed that the positive rate of primary astrocytes was nearly 100%. The results of Western blotting and reverse transcription-PCR showed that expressions of brain-derived neurotrophic factor and vascular endothelial growth factor were significantly increased in 100 mg/L and 1 000 mg/L groups compared with the control, 1 mg/L and 10 mg/L groups (P < 0.05). Double-labeling immunofluoresence also showed that, under the effect of 1 000 mg/L hyaluronan, the expressions of brain-derived neurotrophic factor and vascular endothelial growth factor in astrocytes were strongly increased compared with the control group. It is confirmed that small molecular-weight hyaluronan can induce astrocytes to up-regulate expressions of brain-derived neurotrophic factor and vascular endothelial growth factor.

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